, Functional proteomic analysis reveals sex-dependent differences in structural and energy-producing myocardial proteins in rat model of alcoholic cardiomyopathy, The Expression of PTHLH in Human Gastric Mucosa Enterochromaffin-Like Cells,
In order to demonstrate the involvement of PTHrP in the adaptation of pressure overload we used a well established model of pressure overload, namely thoracic aortic constriction (TAC), and blocked the activity of PTHrP by application of a PTHrP receptor antagonist (PTHrP‐AG), PTHrP(7–34), via osmotic minipumps.
Need help? After the haemodynamic measurements the animals were sacrificed by exsanguination, the hearts were excised and all external fluids were completely removed before weighing.
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One of the key players involved in the transition of hypertrophy to heart failure is TGF‐βInitially, the PTHrP receptor antagonist used in this study was used as a classical PTH/PTHrP receptor antagonist. Please read our short guide
In both TAC groups, ANP mRNA expression was increased compared to sham operated animals, but there was no difference between TAC groups (Table In contrast to markers of myocardial hypertrophy, there were significant differences in left ventricular function between antagonist treated and non‐treated animals. Cells were cultured in the presence of PTHrP, PTHrP antagonist, or vehicle for 24 h.Cell shortening was determined as described before in greater detail At the end of the experiments, hearts were removed and blood samples were taken. The mice used in this study did not differ in regard to body weight (Table The results on gross characterization of myocardial hypertrophy were confirmed on the basis of hypertrophy‐associated gene transcription.
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Cell shortenings are normalized to diastolic cell lengths.Identification of a novel 17,000‐dalton parathyroid hormone‐like adenylate cyclase‐stimulating protein from a tumor associated with humoral hypercalcemia of malignancyParathyroid hormone‐related protein purified from a human lung cancer cell lineParathyroid hormone‐like protein from human renal carcinoma cells.
Where more than two groups were compared, analysis of variance was performed, using a Bonferroni post hoc analysis. 1 Studies evaluating pathophysiology, disease course, and therapeutic options for cardiac arrhythmias have been performed predominantly in male patients.
1.
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This study has excluded the possibility that the observed ventricular up‐regulation of PTHrP is just an epiphenomenon.
This study was therefore conducted to either prove or disprove such a relationship.
Isolated ventricular cardiomyocytes from mice were exposed to either PTHrP(1–34) or PTHrP(7–34), the antagonist used in the The present study was designed to evaluate the functional consequences of pressure‐dependent increased myocardial PTHrP expression.
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Preparation was performed under isoflurane (2.5% vol/vol), and recordings under isoflurane 1% (vol/vol). Sex differences have the potential to impact diagnostic and therapeutic interventions in a wide variety of medical conditions, and cardiac arrhythmias are no exception.
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